General Cell Lines and Cell Cultures
Eurofins Discoverx PathHunter U2OS mFCGR2B Clustering Cell Line, 2 vials. (93-1139C3)
The Path Hunter mFcyRIIb clustering cell line is a stable, engineered cell line designed to increase signal produced from a Checkpoint Cell Line via antibody-mediated crosslinking of the receptors. This product is a companion product used to determine cross-linking based increases in potency of agonist antibodies. The cell line included overexpresses mFcyRIIb Fcg receptor. When co-cultured with a Checkpoint Cell Line in the presence of agonist antibody, the Fcg receptor binds to the antibody, inducing receptor clustering. The induced receptor clustering leads to increased activation and downstream signaling, leading to increased EFC signal production. This stable modified cell line is provided as two vials of cryopreserved cells. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and datasheet, visit www.discoverx.com/checkpoint.
Sartorius Human Multi Ab Internalization
No wash protocol- fast easy antibody-labeling method optimal for screening antibody internalization.High throughput evaluation of antibody internalization- Assay designed to measure internalization of a large number of antibodies in 96- and 384-well plate formats using 10 L of cells and 10 L of labeled antibody kit .Simultaneously measure antibody internalization cell specificity and cell health within the same well.PrincipleAntibodies are easily labeled with Intellicyt Antibody Internalization Reagent a novel pH sensitive dye.
Biochain Institute Inc Paraffin Tissue Section Panel - Human Tumor Same Tumor Type, Different Donors (5 tissues), 5 slides/PK
BioChain's tissue products are based on the sample repository network established following the IRB-approved ethical standard and procedures.The tissues are immediately put in liquid nitrogen after being excised, and then identified by a board-licensed pathologist. Patient demographic and disease related history data are available. he tissue sections are approximately 5-10 um thick.
Eurofins Discoverx PathHunter U2OS mPD-L1 Ligand Cell Line, 2 vials. (93-1159C3)
The PathHunter mPD-L1 Ligand cell line is a stable, engineered cell line designed as a companion product for use with mPD-1 Checkpoint Signaling Cell Lines. When co-cultured, the mPD-L1 expressed in this cell line will induce activity of mPD-1 receptors, which may be assayed through SH2 domain protein recruitment. The cell line included overexpresses mPD-L1 cell surface ligand. When co-cultured with the mPD-1 Checkpoint Signaling Cell Line, the mPD-L1 ligand binds to the checkpoint receptor, inducing receptor activity. This receptor activity leads to recruitment of the relevant SH2 domain protein to the checkpoint receptor inside the receptor cell line and production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and datasheet, visit www.discoverx.com/checkpoint.
Eurofins Discoverx PrecisION hCav3.2 Recombinant Stable Cell Line, 2 vials. (CYL3075)
The PrecisION hCav3.2 Recombinant Stable Cell Line is a stable, engineered cell line for use in studying Cav3.2 ion channels, including drug candidates which affect channel function. This cell line enables assessment of ion channel function via detection of electrophysiological signals. The included cell line overexpresses Cav3.2 ion channels. Upon ligand/agonist induced channel activation, response can be detected via automated or manual electrophysiological techniques. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/ion-channels.
ATCC Salmonella enterica; subsp. enterica; serovar Bispebjerg
Bacteriophage host. Used as a control strain for the detection of Salmonella and in the examination of dairy products.
ATCC Pfizer Pneumococcal polysaccharide powder Type 5 (d5) 200mg
Purified pneumococcal polysaccharide type 5 is manufactured by Pfizer, Inc., for small-quantity distribution by ATCC. The pneumococcal powder type 5 (200 mg) is a component of the current 13-valent vaccine against Streptococcus pneumoniae. The pneumococcal polysaccharide type 5 can be used to assay for type-specific antibodies, research immune responses, and study immunodeficiencies.
Eurofins Discoverx PathHunter U2OS DRD3 β-Arrestin Cell Line, 2 vials. (93-0591C3)
The PathHunter DRD3 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. dopamine) based activation of DRD3 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged DRD3 and EA-tagged β-Arrestin-2. Activation of DRD3 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Eurofins Discoverx cAMP Hunter CHO-K1 CALCRL-RAMP3 Gs Cell Line, 2 vials. (95-0169C2)
The cAMP Hunter CALCRL-RAMP3 Gs cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. adrenomedullin) based activation of CALCRL GPCR activity via detection of increasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type CALCRL, a naturally Gs coupled GPCR, alongside modulator protein RAMP3. Upon activation of CALCRL, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. This stable modified cell line is provided as two vials of cryopreserved cells. This product expresses CALCRL alongside the receptor modifying protein RAMP3, which increases activity of the CALCRL receptor For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
ACROBiosystems CHO/Human CEACAM5 Stable Cell Line Development Service
Carcinoembryonic antigen-related cell adhesion molecule 5 (CEACAM5) is also Carcinoembryonic antigen (CEA), Meconium antigen 100, CD antigen CD66e, CEACAM5 belongs to the immunoglobulin superfamily and CEA family. CEACAM5 contains seven Ig-like (immunoglobulin-like) domains. CEACAM5 is homodimer protein which binding of E.coli Dr adhesins leads to dissociation of the homodimer. CEACAM5 is cell surface glycoprotein that plays a role in cell adhesion and in intracellular signaling. CEACAM5 is receptor for E.coli Dr adhesins.
Lonza Walkersville Mouse C57 Brain Striatum Neuro
Ready-to-use Clonetics Mouse C57 Brain Striatum Neurons (M-CP-302) are isolated from mouse C57 black brain (E14 15) These are sold as cell suspensions of high quality primary embryonic brain neuronal cells (including glia) prepared by standardized methods and are ready for immediate culture Each vial of striatum cells contains approximately 4 million viable cells This will seed into 6 or more wells of a 24-well plate and 30 or more wells of a 96-well plate using the recommended plating densities and medium After approximately 4 days in culture the cells will form a neurite network and by the 7th day debris will be minimal Recommended medium PNGM BulletKit (CC-4461)
Eurofins Discoverx ChemiBrite EP3 Prostanoid Receptor Stable Cell Line, 2 vials. (HTS092L)
The ChemiBrite EP3 Prostanoid Receptor cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. prostaglandin E2) based activation of PTGER3 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses PTGER3 alongside exogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. ChemiBrite cells also express a clytin variant, allowing for luminescent as well as fluorescent signal detection. Upon activation of PTGER3, the resulting calcium activity can be assayed in either detection mode. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, visit www.discoverx.com/targets/gpcr-target-biology.
Eurofins Discoverx PathHunter RXFP3 Total Internalization U2OS Cell Line, 2 vials. (93-0948C3)
The PathHunter RXFP3 Total Internalization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line uses EFC technology to measure ligand-induced internalization of RXFP3 via detection of localization to the early endosome. The included cell line overexpresses ProLink-tagged RXFP3 and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the RXFP3 receptor to the early endosome leads to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
ATCC MCF 10A Ecadherin; EmGFP Reporter; Breast Human
For-profit organizations: For every order of this item, you must provide a signed Material Transfer Agreement Addendum for Screening Application. We cannot ship this item until we receive this addendum. The person signing the addendum as the principal investigator must match the end user as listed on the applicable sales order for the item.Email the signed addendum to SalesPermits@atcc.org with a reference to both your account and sales order numbers. Once received, your addendum will be reviewed, and this item will be released for shipment if all requirements are met. Additional fees may apply if this product is being used for a screening use (ATCC ACS-2103F), and these fees will be applied after your order is confirmed. If you need assistance with your order, please contact our Customer Care team.
Biochain Institute Inc Frozen Tissue Section - Alzheimer's Disease: Brain: Medulla oblongata, 5 slides/PK
BioChain's tissue products are based on the sample repository network established following the IRB-approved ethical standard and procedures. BioChain's Frozen Tissue Slides are ideal for rapidly identifying cellular localization of RNA or protein. A single human adult normal tissue with 5-10 um thickness is mounted on a positively charged glass slide. The slides are fixed and dehydrated with acetone for consistent results with in situ hybridization and immunohistochemistry. More organs may be available upon request.